PR-619 is a non-selective reversible inhibitor of deubiquitinylating enzymes (DUBs) with EC50 of 1-20 μM in cell-free assays. PR-619 activates autophagy.

 

Brand: BCM

 

Target: USP4:3.93 μM(EC50), JOSD2:1.17 μM(EC50), UCH-L3:2.95 μM(EC50), SENP6 core:2.37 μM(EC50), USP8:4.90 μM(EC50)

 

Signaling Pathways: Apoptosis; Autophagy; Cell Cycle/Checkpoint; DNA Damage/DNA Repair; Ubiquitination

 

In Vivo: PR-619 increased global protein polyubiquitination in HEK293T cells in a dose - and time-dependent manner (20 to 150 μM,0.5 to 20 h). The PR619 treatment causes upregulation of the PolyB chain connecting K48 and K63. PR-619 induced HCT116 cell death with EC50 of 6.3 μM.

 

Melting Point: 210℃

 

Boiling Point: 406.0±45.0 °C(Predicted)

 

pKa: -0.19±0.50(Predicted)

 

Solubility: Soluble in DMSO. Insoluble in Water; insolluble in Ethanol.

 

GHS: GHS05,GHS07

 

Isomeric SMILES: C1=C(C(=NC(=C1SC#N)N)N)SC#N  

 

InChIKey: ZXOBLNBVNROVLC-UHFFFAOYSA-N  

 

InChI: InChI=1S/C7H5N5S2/c8-2-13-4-1-5(14-3-9)7(11)12-6(4)10/h1H,(H4,10,11,12)

 

Attribute
[Chem Name]	(2,6-diamino-5-thiocyanatopyridin-3-yl) thiocyanate
[Synonym]	PR-619; PR619
[CAS No.]	2645-32-1
[EINECS]	200-256-5
[MDL No.]	MFCD00830384
[Formula]	C7H5N5S2
[Molecular]	223.28
[Form]	White to beige powder
[Storage]	Sealed in dry, 2-8°C

Goods Tag