Forskolin (Colforsin) is a ubiquitous eukaryotic adenylate cyclase (AC) activator in a wide variety of cell types and is commonly used to increase cAMP levels in cell physiology studies. Forskolin also stimulated the activity of PXR and FXR to induce autophagy.

 

Brand: BCM

 

Target: FXR; Adenylyl cyclase; AChR; Autophagy

 

Signaling Pathways: Autophagy; Metabolism; Neuroscience

 

In Vitro: Forskolin (Fsk) treatment inhibited the proliferation of both Kit 225 and MT-2 cells in a dose-dependent manner with an IC50 equal to ～5 μM Forskolin. Fsk treatment (10–100 μM) increased cAMPi levels ～5- to 20-fold above basal levels, which reached maximum levels between 50–100 μM Fsk. Forskolin binds to adenylyl cyclase in membranes from stably transfected Sf9 cells expressing type 1 adenylyl cyclase with an IC50 value of 41 nM and demonstrates an EC50 value of 0.5 μM in an activation assay assessing formation of cAMP from ATP. "VC6T" [VPA, CHIR99021 (CHIR), 616452, Tranylcypromine] plus Forskolin (VC6TF) induced some GFP-positive clusters expressing E-cadherin.

 

Melting Point: 282-232 °C

 

Specific Rotation: D25 -26.19° (c = 1.68 in CHCl3)

 

Flashing Point: 172℃

 

pKa: 11.00±0.70(Predicted)

 

Solubility: Soluble in DMSO; Soluble in Ethanol. Insoluble in Water.

 

GHS: GHS07

 

Isomeric SMILES: CC(=O)O[C@H]1[C@H]([C@@H]2[C@]([C@H](CCC2(C)C)O)([C@@]3([C@@]1(O[C@@](CC3=O)(C)C=C)C)O)C)O  

 

InChIKey: OHCQJHSOBUTRHG-KGGHGJDLSA-N  

 

InChI: InChI=1S/C22H34O7/c1-8-19(5)11-14(25)22(27)20(6)13(24)9-10-18(3,4)16(20)15(26)17(28-12(2)23)21(22,7)29-19/h8,13,15-17,24,26-27H,1,9-11H2,2-7H3/t13-,15-,16-,17-,19-,20-,21+,22-/m0/s1

 

Attribute
[Chem Name]	[(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-3-ethenyl-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-5,6,6a,8,9,10-hexahydro-2H-benzo[f]chromen-5-yl] acetate
[Synonym]	Forskolin; Colforsin; Coleonol
[CAS No.]	66575-29-9
[EINECS]	266-410-9
[MDL No.]	MFCD00082317
[Formula]	C22H34O7
[Molecular]	410.5
[Form]	Off-white powder
[Storage]	Sealed in dry. Store in freezer, under -20°C

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