JW74 is a specific inhibitor of classical Wnt signaling, with an IC50 of 790 nM in ST-Luc assay. It inhibits the growth of tumor cells in mouse xenotransplantation models of colorectal cancer and in ApcMin mice.

 

Brand: BCM

 

Target: Wnt/beta-catenin

 

Signaling Pathways: Cytoskeletal Signaling; Stem Cells

 

In Vitro: Ki-67 expression is reduced from 97.5% in DMSO-treated cells to 86.7% in JW74-treated cells. Relative to DMSO-treated cells, the cellular viability of U2OS cells treated for 72 h treatment with 10 μM JW74 is reduced to 80%. The effect of tankyrase inhibition on cellular viability is tested by performing an MTS assay. Flow cytometry is also performed to determine the expression marker Ki-67 in U2OS following 48 h treatment with DMSO or 10 uM JW74. JW74 displays a reduction of canonical Wnt signaling in the ST-Luc assay (IC50: 790 nM) 

 

Boiling Point: 699.4±65.0 °C(Predicted)

 

pKa: 1.63±0.10(Predicted)

 

Solubility: Soluble in DMSO; Soluble in Ethanol. Insoluble in Water. 

 

Isomeric SMILES: CC1=CC=C(C=C1)C2=NOC(=N2)CSC3=NN=C(N3C4=CC=C(C=C4)OC)C5=CC=NC=C5  

 

InChIKey: KRIKILRRJCIWNB-UHFFFAOYSA-N  

 

InChI: InChI=1S/C24H20N6O2S/c1-16-3-5-17(6-4-16)22-26-21(32-29-22)15-33-24-28-27-23(18-11-13-25-14-12-18)30(24)19-7-9-20(31-2)10-8-19/h3-14H,15H2,1-2H3

 

Attribute
[Chem Name]	5-[[4-(4-methoxyphenyl)-5-pyridin-4-yl-1,2,4-triazol-3-yl]sulfanylmethyl]-3-(4-methylphenyl)-1,2,4-oxadiazole
[Synonym]	JW74
[CAS No.]	863405-60-1
[MDL No.]	MFCD07048962
[Formula]	C24H20N6O2S
[Molecular]	456.52
[Form]	White to beige powder
[Storage]	Sealed in dry. Stored at -20°C.

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